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Types of Tissue Culture (Part- 04)= Suspension Culture (HINDI) By Solution Pharmacy

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Definition:
Suspension culture is a type of culture in which single cells or small aggregates of cells mul­tiply while suspended in agitated liquid medium. It is also referred to as cell culture or cell suspen­sion culture.

Principle:
The cultivation of free cells as well as small cell aggregates in a chemically de­fined liquid medium as a suspension was initi­ated to study the morphological and biochemi­cal changes during their growth and developmen­tal phases.To achieve an ideal cell suspension, most commonly a friable callus is transferred to agitated liquid medium where it breaks up and readily disperses. After eliminating the large cal­lus pieces, only single cells and small cell aggre­gates are again transferred to fresh medium and after two or three weeks a suspension of actively growing cells is produced.

The culture of single cells and cell aggregates in mov­ing liquid medium can be handled as the culture of microbes. Movement of the cells in relation to nutrient medium facilitates gaseous exchange, removes any polarity of the cells due to gravity and eliminates the nutrient gradients within the medium and at the surface of the cells.

Importance of Cell Suspension Culture:
Sus­pension culture derived from medicinally impor­tant plants can be studied for the production of secondary metabolites such as alkaloids and a considerable amount of industrial effort is being placed on the exploitation and expansion of this area. Mutagenesis studies may be facilitated by the use of cell suspension cultures to produce mutant cell clones from which mutant plants can be raised.

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Видео Types of Tissue Culture (Part- 04)= Suspension Culture (HINDI) By Solution Pharmacy канала Solution- Pharmacy
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21 июня 2019 г. 20:01:09
00:26:05
Яндекс.Метрика