Different Types of Plant Tissue Culture (Part-01) (HINDI) By Solution Pharmacy

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Plant Tissue culture
Plant tissue culture has a great significance in plant biotechnology, especially in the crop improvement programmes. The term tissue culture may be defined as the process of the in-vitro culture of explants (pieces of living differentiated tissues) in a nutrient medium under aseptic conditions
The following points highlight the top eight types of tissue culture. The types are:
1. Seed Culture 2. Embryo Culture 3. Meristem Culture 4. Bud Culture 5. Protoplast Culture
6. Root tip Culture 7. Anther/ Pollen Culture 8. Complete flower Culture 9. Ovary Culture
10. Hairy Root culture 11. Callus Culture 12. Cell Suspension Culture.
1. Seed Culture:
Seeds may be cultured in vitro to generate seedlings or plants. It is the best method for raising the sterile seedling.
2. Embryo Culture:
Embryo culture is the sterile isolation and growth of an immature or mature embryo in vitro with the goal of obtaining a viable plant.
3. Meristem Culture:
The apical meristem of shoots of angiosperms and gymnosperms can be cultured to get the disease-free plants.
4. Bud Culture:
Buds contain quiescent or active meristems in the leaf axils, which are capable of growing into a shoot.
5. Callus Culture:
Callus is basically more or less un-organised dedifferentiated mass of cells arising from any kind of explant under in vitro cultural conditions.
6. Cell Suspension Culture:
The growing of individual cells that have been obtained from any kind of explant tissue or callus referred to as cell suspension culture.
7. Anther Culture:
An important aspect of plant tissue culture is the haploid production by another culture or pollen culture which was first established
8. Protoplast Culture:
It is the culture of plant protoplasts i.e., culture of cells devoid of cell wall. Isolated protoplasts are usually cultured in either liquid or semisolid agar media plates.

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