Applications of Plant Tissue Culture (HINDI) By Solution Pharmacy

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Applications of plant tissue culture
1. Production of Useful Bio-chemicals (Secondary Metabolites)
Production of many com¬pounds other than food such as medicines, pigments, vitamins, hormones, flavoring agents, latex and tanins. Produce specialized bio- chemicals found in the intact parent, including alkaloid such as nicotine, atropine, ephedrine, caffeine and codeine and their precursors and derivatives.
2. Micro Propagation/Clonal Propagation
The regeneration of whole plant through tissue culture is popularly called “micro-propagation”. This is a technique where a callus mass has been initiated from a single explant taken from any living part of a donor plant and within very short time and space, a large number of plantlets can be produced from such callus tissue.
3. Biotranformation
4. Immobilization of Plant cell
1. Production of Genetically Variable Plants (Somaclonal variation): The chromosomal instability in the cultured cells play an important role in polyploidization of cells and genetically variable plants can be raised from such polyploidized cells by subsequent micro-propagation.
2. Plant Breeding, Plant Improvement and Plant Tissue Culture
3. Preservation of Plant Genetic Resources or Gene Conservation Banks the conser-vation of plant genetic resources. The plant materials can be stored in liquid nitrogen for desirable period. This technique could be par¬ticularly valuable for storage of any germplasm which needs to be maintained in a clonal form. This technique is known as cryopreservation or freeze preservation of tissue or cell.
4. Development of Synthetic Seed.
5. Importance of Tissue Culture in Biotechnology
Mutagenesis and selection of cell lines in cell suspension culture.Transplantation of foreign genetic material in protoplasts by means of genetic engineering and
6. Somatic Hybridization
Somatic hybridization by the fusion of distantly related plant protoplast. These methods are the most widely used for crop improvement.

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