Visualizing tissues and immune cells in 3D

Animation of a 3D image of intact (non-sectioned) mouse lymph node imaged using the LBS-developed method of Ce3D clearing and staining. Cyan = B220 staining of B cells. Yellow = CD31 staining of vascular endothelial cells. White = LYVE-1 staining of lymphatic endothelial cells. Green = CD8 staining of T cells and CD8+ dendritic cells. Red = CD169 staining of macrophages. Image first shows vascular tree, then adds B cell follicles, then shows in silico computer sectioning of the entire lymph node.

W. LI, M. Gerner, and R. N. Germain, unpublished.

Видео Visualizing tissues and immune cells in 3D канала NIAID