Serial dilutions and pour plate technique
Serial dilution involves repeatedly mixing known amounts of source culture with (sterilised) liquid. 1 ml added to 9 ml gives a 10-fold dilution; 1 ml added to 99ml gives a 100-fold dilution. When fixed amounts of this dilution series are mixed with an appropriate agar and incubated, then different numbers of colonies will be obtained. By working back from an easily counted plate and using the appropriate dilution factor, the number of micro-organisms in the original source culture can be calculated.
In a pour plate technique, a small amount of inoculum from a broth culture is added by pipette to the centre of a Petri dish. Cooled, but still molten, agar medium in a test tube or bottle is then poured into the Petri dish. The dish is then rotated gently, or moved back and forth, to ensure that the culture and medium are thoroughly mixed and the medium covers the plate evenly. Pour plates allow micro-organisms to grow both on the surface and within the medium. Most of the colonies grow within the medium and are small in size and may be confluent. The few colonies that grow on the surface are of the same size and appearance as those on a streak plate.
Видео Serial dilutions and pour plate technique канала Microbial Zoo
In a pour plate technique, a small amount of inoculum from a broth culture is added by pipette to the centre of a Petri dish. Cooled, but still molten, agar medium in a test tube or bottle is then poured into the Petri dish. The dish is then rotated gently, or moved back and forth, to ensure that the culture and medium are thoroughly mixed and the medium covers the plate evenly. Pour plates allow micro-organisms to grow both on the surface and within the medium. Most of the colonies grow within the medium and are small in size and may be confluent. The few colonies that grow on the surface are of the same size and appearance as those on a streak plate.
Видео Serial dilutions and pour plate technique канала Microbial Zoo
Показать
Комментарии отсутствуют
Информация о видео
Другие видео канала
Gram Staining Procedure Animation Microbiology - Principle, Procedure, InterpretationSerial Dilution and Plate CountsSerial Dilutions of a Bacterial CultureStreak Plate Technique for The Isolation of Pure Culture_A Complete Procedure (Microbiology)Spread Plates | Colony Counts (CFUs/mL) and Difference from Streak PlatesAseptic TechniqueSerial dilutions lessonPure culture isolation techniquesHow to Count and Pick ColoniesDilutions - Part 3 of 4 (Calculating Colony Forming Units/ml)Culture MediaGram StainingMedia PrepPour PlatingViable cell countPour Plate Technique for Colony Counting_A Complete Procedure (Microbiology)Serial dilution technique and its Calculation (Pour plate and Spread plate technique)How to create serial dilutionsОпределение чувствительности микроорганизмов к антибактериальным препаратамHow to Make LB, a Standard Bacterial Growth Medium