Preparing sterile ampicillin stock solutions

Hello Everyone,

I’ve created these videos primarily as instructional aides for new students, interns, and trainees in my research group. While many of the methods are generally applicable, I am of course producing these demonstrations with the materials, reagents, and equipment that are available in our lab. Also, I’ll occasionally reference the locations of items in our lab.

In this video, I go over how to prepare sterile ampicillin stock solutions. Evidently, this same procedure can be applied to a variety of other stock solutions (IPTG, glucose, etc.). It’s worth noting that a number of other antibiotics (such as tetracycline and chloramphenicol) only exhibit reasonable solubility in ethanol or other organic solvents. It is not necessary to filter-sterilize these ethanol / antibiotic stock solutions.

Some labs do not allow the use of Bunsen burners to maintain a sterile field, and that’s fine if this is the case in your lab. Since we have Bunsen burners, it’s a good idea to try to open sterile containers near a flame. Because of the camera angle, it sometimes looks like my hands are pretty far from the flame. They are not. I am keeping open containers within six inches of the flame whenever possible.

About me: I am currently (at the time of uploading this video) a doctoral student in the Chemical and Biomolecular Engineering department at The Ohio State University. My advisor is Dr. David Wood, and our research group specializes in protein engineering and downstream processing for the biotechnology industry. I’m also very new at producing instructional videos so I apologize in advance if these are not of professional quality. For instance, in my first few videos I did not know how to get rid of the timestamp (which is wrong), however I posted them anyways.

Please keep comments civil and apolitical. You are of course welcome to ask questions, however it is unlikely that I will answer them if you are not a member of my research group.

Thanks for watching,
Steven

Видео Preparing sterile ampicillin stock solutions канала Steven Cummings