Inoculating small-scale E. coli cultures (starting miniprep cultures)

Hello Everyone,

I’ve created these videos primarily as instructional aides for new students, interns, and trainees in my research group. While many of the methods are generally applicable, I am of course producing these demonstrations with the materials, reagents, and equipment that are available in our lab. Also, I’ll occasionally reference the locations of items in our lab.

In this video, I go over how to prepare small-scale E. coli cultures like you would for minipreps or starter cultures. I started these preps at about 02h00 so I’m a bit tired (3*18 does not equal 68… don’t worry; I catch my mistake), but this video should give you a general idea of the different ways to inoculate these cultures and how to handle a culture tube around a flame.

A couple notes: Because of the camera angle it sometimes looks like what I’m holding is fairly far from the flame. This is not the case. You should try to keep open culture tubes and media bottles within 4-6 horizontal inches from the flame. Similarly, despite appearances, I’m not actually spraying ethanol on my hands 2” away from the flame.

About me: I am currently (at the time of uploading this video) a doctoral student in the Chemical and Biomolecular Engineering department at The Ohio State University. My advisor is Dr. David Wood, and our research group specializes in protein engineering and downstream processing for the biotechnology industry. I’m also very new at producing instructional videos so I apologize in advance if these are not of professional quality. For instance, in my first few videos I did not know how to get rid of the timestamp (which is wrong), however I posted them anyways.

Please keep comments civil and apolitical. You are of course welcome to ask questions, however it is unlikely that I will answer them if you are not a member of my research group.

Thanks for watching,
Steven

Видео Inoculating small-scale E. coli cultures (starting miniprep cultures) канала Steven Cummings