What are restriction enzymes
For more information, log on to-
http://shomusbiology.weebly.com/
Download the study materials here-
http://shomusbiology.weebly.com/bio-materials.html
Source of the article published in description is Wikipedia. Thanks to original content developers.
Link- http://en.wikipedia.org/wiki/Main_Page
A restriction enzyme (or restriction endonuclease) is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites.[1][2][3] Restriction enzymes are commonly classified into three types, which differ in their structure and whether they cut their DNA substrate at their recognition site, or if the recognition and cleavage sites are separate from one another. To cut DNA, all restriction enzymes make two incisions, once through each sugar-phosphate backbone (i.e. each strand) of the DNA double helix.
These enzymes are found in bacteria and archaea and provide a defense mechanism against invading viruses.[4][5] Inside a prokaryote, the restriction enzymes selectively cut up foreign DNA in a process called restriction; while host DNA is protected by a modification enzyme (a methylase) that modifies the prokaryotic DNA and blocks cleavage. Together, these two processes form the restriction modification system.[6]
Over 3000 restriction enzymes have been studied in detail, and more than 600 of these are available commercially.[7] These enzymes are routinely used for DNA modification in laboratories, and are a vital tool in molecular cloning.[8][9][10]
Видео What are restriction enzymes канала Shomu's Biology
http://shomusbiology.weebly.com/
Download the study materials here-
http://shomusbiology.weebly.com/bio-materials.html
Source of the article published in description is Wikipedia. Thanks to original content developers.
Link- http://en.wikipedia.org/wiki/Main_Page
A restriction enzyme (or restriction endonuclease) is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites.[1][2][3] Restriction enzymes are commonly classified into three types, which differ in their structure and whether they cut their DNA substrate at their recognition site, or if the recognition and cleavage sites are separate from one another. To cut DNA, all restriction enzymes make two incisions, once through each sugar-phosphate backbone (i.e. each strand) of the DNA double helix.
These enzymes are found in bacteria and archaea and provide a defense mechanism against invading viruses.[4][5] Inside a prokaryote, the restriction enzymes selectively cut up foreign DNA in a process called restriction; while host DNA is protected by a modification enzyme (a methylase) that modifies the prokaryotic DNA and blocks cleavage. Together, these two processes form the restriction modification system.[6]
Over 3000 restriction enzymes have been studied in detail, and more than 600 of these are available commercially.[7] These enzymes are routinely used for DNA modification in laboratories, and are a vital tool in molecular cloning.[8][9][10]
Видео What are restriction enzymes канала Shomu's Biology
Показать
Комментарии отсутствуют
Информация о видео
Другие видео канала
![Types of restriction enzyme](https://i.ytimg.com/vi/O7S_4iuxdWc/default.jpg)
![Recombinant DNA technology | DNA Vectors | Cloning Vector And Expression Vector](https://i.ytimg.com/vi/VXkw_U6mJpc/default.jpg)
![Gel electrophoresis procedure explained | agarose gel electrophoresis of DNA](https://i.ytimg.com/vi/aS-LwdUAk4Q/default.jpg)
![Genetic Markers | RAPD, RFLP, AFLP](https://i.ytimg.com/vi/JVM4LpCuT7g/default.jpg)
![Gene Library | Genomic Library and cDNA Library](https://i.ytimg.com/vi/fmMp6avlB6I/default.jpg)
![PCR (polymerase chain reaction) in detail](https://i.ytimg.com/vi/vi7MeqD2_FY/default.jpg)
![DNA cloning](https://i.ytimg.com/vi/MIfDx417SDs/default.jpg)
![Restriction enzyme digestion (Isochizomer, neoschizomer and isocaudamer)](https://i.ytimg.com/vi/RlcM7KAzv3s/default.jpg)
![DNA ligase](https://i.ytimg.com/vi/YjeHRWW1oKw/default.jpg)
![RESTRICTION ENZYMES](https://i.ytimg.com/vi/rhd_fBPyzSM/default.jpg)
![DNA methylation](https://i.ytimg.com/vi/JuQatrVRjBM/default.jpg)
![Properties of restriction enzymes](https://i.ytimg.com/vi/aUMN0a5XpsU/default.jpg)
![Northern blotting technique](https://i.ytimg.com/vi/GBmFaf2krYY/default.jpg)
![PCR - Polymerase Chain Reaction Simplified](https://i.ytimg.com/vi/uKeMiAZ8Zu4/default.jpg)
![Cloning vector](https://i.ytimg.com/vi/jZBwtywWx7o/default.jpg)
![DNA sequencing methods](https://i.ytimg.com/vi/I5pwZTJMVCQ/default.jpg)
![Restriction mapping problems tutorial](https://i.ytimg.com/vi/GgaMq69zZ2M/default.jpg)
![Difference between linker and adaptor DNA](https://i.ytimg.com/vi/scpNAdFC8QU/default.jpg)
![Southern blotting](https://i.ytimg.com/vi/KVyPNv8qeNU/default.jpg)
![Recombinant DNA technology lecture | basics of recombinant DNA](https://i.ytimg.com/vi/3Z5j0xl8knY/default.jpg)