Southern blotting
Southern blotting - This lecture explains about the southern blotting procedure and technique to identify specific DNA fragment from a mixture of DNA molecules separated by the Agarose gel electrophoresis. Southern blotting step by step explanation is provided to understand the procedure of the DNA separation and identification. Southern blotting application is also explained in this video.
Southern blotting consists of three different stages -
1. First is the isolation of DNA from the target cell.
2. Then the treatment of restriction digestion of the DNA to generate DNA fragments
3. Then the separation of the DNA fragments using Agarose gel electrophoresis
4. Transfer of the Agarose gel DNA bands to a nitrocellulose Membrane filter. This filter paper after the DNA transfer is known as southern blot.
5. Then the use of complementary DNA probe to bind with the target DNA that will indicate the presence of target sequence by radioactivity or colorize trick expression. Southern blotting was invented by EM Southern on 1975 at it was named after him to identify DNA using blotting technique.
For more information, log on to-
http://www.shomusbiology.com/
Get Shomu's Biology DVD set here-
http://www.shomusbiology.com/dvd-store/
Download the study materials here-
http://shomusbiology.com/bio-materials.html
Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology-
Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store
Shomu’s Biology assignment services – www.shomusbiology.com/assignment -help
Join Online coaching for CSIR NET exam – www.shomusbiology.com/net-coaching
We are social. Find us on different sites here-
Our Website – www.shomusbiology.com
Facebook page- https://www.facebook.com/ShomusBiology/
Twitter - https://twitter.com/shomusbiology
SlideShare- www.slideshare.net/shomusbiology
Google plus- https://plus.google.com/113648584982732129198
LinkedIn - https://www.linkedin.com/in/suman-bhattacharjee-2a051661
Youtube- https://www.youtube.com/user/TheFunsuman
Thank you for watching southern blotting lecture.
Видео Southern blotting канала Shomu's Biology
Southern blotting consists of three different stages -
1. First is the isolation of DNA from the target cell.
2. Then the treatment of restriction digestion of the DNA to generate DNA fragments
3. Then the separation of the DNA fragments using Agarose gel electrophoresis
4. Transfer of the Agarose gel DNA bands to a nitrocellulose Membrane filter. This filter paper after the DNA transfer is known as southern blot.
5. Then the use of complementary DNA probe to bind with the target DNA that will indicate the presence of target sequence by radioactivity or colorize trick expression. Southern blotting was invented by EM Southern on 1975 at it was named after him to identify DNA using blotting technique.
For more information, log on to-
http://www.shomusbiology.com/
Get Shomu's Biology DVD set here-
http://www.shomusbiology.com/dvd-store/
Download the study materials here-
http://shomusbiology.com/bio-materials.html
Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in YouTube. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology-
Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store
Shomu’s Biology assignment services – www.shomusbiology.com/assignment -help
Join Online coaching for CSIR NET exam – www.shomusbiology.com/net-coaching
We are social. Find us on different sites here-
Our Website – www.shomusbiology.com
Facebook page- https://www.facebook.com/ShomusBiology/
Twitter - https://twitter.com/shomusbiology
SlideShare- www.slideshare.net/shomusbiology
Google plus- https://plus.google.com/113648584982732129198
LinkedIn - https://www.linkedin.com/in/suman-bhattacharjee-2a051661
Youtube- https://www.youtube.com/user/TheFunsuman
Thank you for watching southern blotting lecture.
Видео Southern blotting канала Shomu's Biology
Показать
Комментарии отсутствуют
Информация о видео
Другие видео канала
![Northern blotting technique](https://i.ytimg.com/vi/GBmFaf2krYY/default.jpg)
![Gel electrophoresis procedure explained | agarose gel electrophoresis of DNA](https://i.ytimg.com/vi/aS-LwdUAk4Q/default.jpg)
![Southern Blot](https://i.ytimg.com/vi/3I9wzwj0b_A/default.jpg)
![Southern Blotting](https://i.ytimg.com/vi/-SaKbLTQ4WI/default.jpg)
![Delta plus variant covid | Will Delta plus variant cause covid third wave in India?](https://i.ytimg.com/vi/S5cCk43iwf8/default.jpg)
![Nucleic Acid Hybridization and Probes](https://i.ytimg.com/vi/29EC7L3t19c/default.jpg)
![Southern Blot Method - Animation video](https://i.ytimg.com/vi/CSrUm-EgTK4/default.jpg)
![Southern blot | Biomolecules | MCAT | Khan Academy](https://i.ytimg.com/vi/Zps2uH8aWVU/default.jpg)
![PCR - Polymerase Chain Reaction Simplified](https://i.ytimg.com/vi/uKeMiAZ8Zu4/default.jpg)
![In Situ Hybridization](https://i.ytimg.com/vi/IeUKt7nGNDA/default.jpg)
![Western Blotting Animation - Part II](https://i.ytimg.com/vi/JcN0EkcHrKk/default.jpg)
![Difference between Southern and northern blotting and western blotting](https://i.ytimg.com/vi/OUlfbGfwdIk/default.jpg)
![Sanger sequencing method - dideoxy sequencing of DNA](https://i.ytimg.com/vi/NX3SLWILPKk/default.jpg)
![Western blot](https://i.ytimg.com/vi/Yh69yHJMWPc/default.jpg)
![Genetic Markers | RAPD, RFLP, AFLP](https://i.ytimg.com/vi/JVM4LpCuT7g/default.jpg)
![ELISA test](https://i.ytimg.com/vi/YJ0-qQslqqQ/default.jpg)
![Western Blotting (Immunoblotting) : Principle and Complete Procedure Explained ! (FL-Immuno/81)](https://i.ytimg.com/vi/Ll_7z4YS2Ak/default.jpg)
![DNA sequencing methods](https://i.ytimg.com/vi/I5pwZTJMVCQ/default.jpg)
![Western blotting technique | principle and step by step procedure](https://i.ytimg.com/vi/N8XbauUsns4/default.jpg)
![PCR in Hindi](https://i.ytimg.com/vi/hb-S2j7s89k/default.jpg)