CHAPTER 4 - Protein Primary Structure and Proteomics

HARPER BIOCHEMISTRY
CHAPTER 4

This discusses the critical importance of proteins in biological systems, highlighting their diverse functions from maintaining cell shape to catalysing reactions and transporting molecules. It explains that to fully understand proteins, they often need to be purified first, outlining various chromatographic techniques like size-exclusion, ion-exchange, hydrophobic interaction, and affinity chromatography, along with electrophoresis methods like SDS-PAGE and isoelectric focusing, used for both purification and analysis. The text also details the historical development of protein sequencing methods, starting with Sanger's approach for insulin, the more practical Edman degradation, and the revolutionary impact of molecular biology and genomics in determining protein sequences by analysing their encoding DNA. Finally, it introduces mass spectrometry (MS) as a powerful, modern technique for protein identification, detecting modifications, and studying the entire complement of proteins, known as the proteome.

Видео CHAPTER 4 - Protein Primary Structure and Proteomics канала MED_NESS