CRISPR Gene Editing: Using CRISPR-Cas9 with the Out of the Blue CRISPR Kit

Follow along with this step-by-step walkthrough of the lacZ gene editing laboratory activity in Bio-Rad’s Out of the Blue #CRISPR Kit. Click “Show More” for chapters and free resources. 👇

00:00 – Introduction
01:22 – lacZ in blue-white screening
02:57 – CRISPR-Cas9 gene editing
05:21 – Laboratory protocol overview and background
08:23 – Experimental setup
09:24 – Example Results
09:55 – Instructor Preparation
10:22 – Student workstation setup
11:17 – Student protocol
20:36 – Additional Information

See the first video in this series CRISPR Explained: Gene Editing History, Technology, and Applications at https://www.youtube.com/watch?v=tNocAc5TKvw

For more videos about bacterial transformation visit the pGLO Bacterial Transformation playlist at https://www.youtube.com/playlist?list=PL7_N-H8d6RiNkwLkza-ZhSdnl12lCXqXv

The 2020 Nobel Prize in Chemistry was awarded for CRISPR. Learn more about the Out of the Blue CRISPR Kit and Genotyping Extension, and download free modeling and bioinformatics activities in the instructor and student guides, at http://bio-rad.com/outoftheblue

Free CRISPR teaching resources at http://bio-rad.com/teachcrispr

See more curated videos on our CRISPR Gene Editing playlist at https://www.youtube.com/playlist?list=PL7_N-H8d6RiOTKL7AIOzP69C8wYWKYOi_

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Видео CRISPR Gene Editing: Using CRISPR-Cas9 with the Out of the Blue CRISPR Kit канала Bio-Rad Explorer