5. How to calculate pI from 3 pKa Values | Amino Acids | Biochemistry

Hello friends,
Here, are a few questions for practice.
Note - If you do not find the exact value in options mark the closest one as correct.

1) The pKa values of Lysine are given below. Calculate the pI
pKa of COOH = 2.2
pKa of NH3 = 9.0
pKA of R- NH3 = 10.0

a. 7.07
b. 9.50
c. 6.16
d. 5.60

2) The three pKa values of histidine are
pKa of COOH = 1.8
pKa of NH3 = 9.3
pKA of R- NH = 6 (Hint - NH in protonated from is NH2+)
Answers
1) b
3) 7.6

Видео 5. How to calculate pI from 3 pKa Values | Amino Acids | Biochemistry канала Manmohan Mitruka