12. Relation b/w Biomass & Substrate Concentration | Bioprocess Technology| Questions in Description

Questions -
Q1. Saccharomyces cerevisiae is cultured in continuous fermentation at a dilution rate of 0.5 per hr. The feed substrate concentration is 10g/l. The Biomass concentration in a chemostat at steady state will be ----- g/l.
The maximum Biomass yield with respect to the substrate is 0.4.
umax= 0.7 per hr
Ks = 0.3g/l
[GATE 2016]

Q2. The maximum concentration expected in a bioreactor with an initial cell concentration of 1.75g/l and initial glucose concentration of 125g/l is________.
Yield with respect to the substrate is 0.6g cell/g substrate. [GATE 2013]

Q3. A microorganism grows in continuous chemostat culture of 60-meter cube working volume. With sucrose as the growth-limiting nutrient at dilution rate of 0.55 per hr. The steady-state biomass concentration is 4.5 kg dry Biomass per metre cube. And the Residual sucrose concentration is 2kg per meter cube. The sucrose concentration in the incoming feed is 10kg per meter cube. What would be the yield of biomass with respect to substrate? [GATE 2011]
a. 0.562
b. 0.462
c. 0.362
d. 0.162

Q4. The volume of chemostat system is 1000L. The feed flow rate to the reactor is 200 litre per hour. And the glucose concentration in the feed is 5g/l. Determine cell and glucose concentration in the effluent of the reactor under steady-state conditions. Use the following constants for the cells.
umax=0.3 per hr ,Ks= 0.1g/l Y(biomass with respect to substrate)=0.4
[GATE 2002]

Q5. The unit of specific substrate consumption rate in a growing culture is
[GATE 2014]
a. g/l.hr
b. g/g.hr
c. g/hr
d. g moles/l.hr

Answers –
1. 3.70 g/liter
2. 76.75 g/liter
3. a
4. Steady-state substrate 0.2 g/l, Cell concentration 1.92g/l
5. b
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Видео 12. Relation b/w Biomass & Substrate Concentration | Bioprocess Technology| Questions in Description канала Manmohan Mitruka