plastic-eating machine: the bacteria that eat PET

In 2016 scientists discovered a species of bacteria that is able to digest PET plastic, which we now know is thanks to an enzyme called PETase. But where did this enzyme come from? Video in collaboration with Lumi at the Benchtop- check out his video here: https://youtu.be/T2kqIHh5w4Y
▫️ written by April and Roshni, narrated & edited by April

▫️information sources:
+Yoshida, S. et. al. (2016). A bacterium that degrades and assimilates poly(ethylene terephthalate). Science https:/doi.org/10.1126/science.aad6359
+Knott, B. et al. (2020). Characterization and engineering of a two-enzyme system for plastics depolymerization, PNAS. https://doi.org/10.1073/pnas.2006753117
+Austin, H. et al. (2018). Characterization and engineering of a plastic-degrading aromatic polyesterase, PNAS. https://doi.org/10.1073/pnas.1718804115
+Newton, M. et al. (2018). Enzyme evolution: innovation is easy, optimization is complicated. Current Opinion in Structural Biology. https://doi.org/10.1016/j.sbi.2017.11.007
+Huang, H. et al. (2015). Panoramic view of a superfamily of phosphatases through substrate profiling, PNAS. https://doi.org/10.1073/pnas.1423570112
+Joo, S. et al. (2018). Structural insight into molecular mechanism of poly(ethylene terephthalate) degradation. Nature Communications. https://doi.org/10.1038/s41467-018-02881-1
+ Machado de Castro, A. et al. (2017). Screening of commercial enzymes for poly(ethylene terephthalate) (PET) hydrolysis and synergy studies on different substrate sources. Journal of Industrial Microbiology and Biotechnology. https://doi.org/10.1007/s10295-017-1942-z
+Tournier, V. et al. (2020).An engineered PET depolymerase to break down and recycle plastic bottles, Nature. https://doi.org/10.1038/s41586-020-2149-4
+'Bakelite: The World’s First Synthetic Plastic'. American Chemical Society National Historic Chemical Landmarks. http://www.acs.org/content/acs/en/education/whatischemistry/landmarks/bakelite.html
+'History and Future of Plastics'. Science History Institute. https://www.sciencehistory.org/the-history-and-future-of-plastics

▫️media sources:
+'The Man in the Doorway' (1957) retrieved from YouTube user A/V Geeks https://youtu.be/i_Xlkc3B1P4
+'Plastics' (1973) retrieved from YouTube user A/V Geeks https://youtu.be/hHKaAR9P2mA?t=2206
+ 'What is an energy landscape? [part 1]' retrieved from YouTube user Bio Comp Pictures https://youtu.be/pM8__r-Kxmo
+molecular simulation of MHETase from Knott et. al (2020).

Funded by: UPMC Hillman Academy & NCI R25 YES

#plastic #evolution #science

Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation, National Institutes of Health or UPMC.

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