A Guide to the Colony Forming Cell Assay: Methods and Tips

This video protocol provides step-by-step instructions on how to consistently perform the Colony Forming Cell (CFC) Assay. Tips are provided throughout the video to help optimize the assay procedure, including tricks to accurately evaluate and score colony formations.

00:10 CFC Assay Overview
00:54 Aliquoting the Media
02:06 Preparing the Cells
04:05 Plating the Cells
05:14 Counting the Cells
05:53 Tips for Colony Identification

FULL WRITTEN PROTOCOLS FOR OUR CFC ASSAYS:
The Human Colony Forming Cell (CFC) Assay
http://www.rndsystems.com/literature_CFC.aspx
The Mouse/Rat Colony Forming Cell (CFC) Assay
http://www.rndsystems.com/literature_MouseMethylcellulose.aspx
The Mouse Pre-B Colony Forming Cell (CFC) Assay
http://www.rndsystems.com/literature_mouse_preb_cfc_protocol.aspx

GUIDES TO CHOOSING METHYLCELLULOSE MEDIA:
For the Human CFC Assay:
http://www.rndsystems.com/product_detail_objectname_choosing_methylcellulose_media_for_the_human_colony_forming_cell_cfc_assay.aspx
For the Mouse CFC Assay
http://www.rndsystems.com/product_detail_objectname_choosing_methylcellulose_media_for_the_mouse_colony_forming_cell_cfc_assay.aspx
For the Rat CFC Assay
http://www.rndsystems.com/product_detail_objectname_choosing_methylcellulose_media_for_the_rat_colony_forming_cell_cfc_assay.aspx

Видео A Guide to the Colony Forming Cell Assay: Methods and Tips канала R&D Systems, a Bio-Techne brand