Apoptosis assay - AnnexinV PI

A common question asked in many research papers - are cancer cells dying after treatment with some drug or other treatment. The dying here is apoptosis - programmed cell death (we will learn the apoptosis in detail in a later unit). There are many techniques used by scientists to quantitate apoptosis. This video covers on type of apoptosis assy: the Annexin V/PI assay. Learning objectives:

Know what Annexin V binds to on a cell, and when that binding can occur.
Know what PI binds to in cell, and when that binding can occur.
Explain the four quandants of a Annexin V/PI assay, relating to Annexin V + or -, PI + or -, and how they represent the state of a cell (alive, early apoptosis, late apoptosis, necrosis)

Видео Apoptosis assay - AnnexinV PI канала Joe DeMasi