Figure 6.2 and 6.3 RNAP holoenzyme binds promoter DNA better than core enzyme
This video describes experiments that showed that the RNA polymerase holoenzyme binds to promoter DNA much better than the core enzyme, suggesting a role of sigma factor for tight binding. It also has a nice description of filter binding to study protein-DNA interactions. Described are figures 6.2 and 6.3 from Molecular Biology by Robert Weaver, edition 5. This video was made for Molecular Biology (MCDB 427) at the University of Michigan.
Видео Figure 6.2 and 6.3 RNAP holoenzyme binds promoter DNA better than core enzyme канала MCDB 427
Видео Figure 6.2 and 6.3 RNAP holoenzyme binds promoter DNA better than core enzyme канала MCDB 427
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Figure 6.26 and 6.27 Footpringing of RNAP and alpha dimers.
RNA Polymerase Melts DNA at the Promoter-Figures 6.15 and 6.16
Fig 15.7 Length of the Poly(A) tail in mRNA
DNA bending--Fig 7 18
Temporal order of RNAP II transcription factor binding Figures 11 1, 11 2 and 11 3
Figure 5.34 Using a CAT Reporter Gene
Figure 15.13 and 15.33 Transcription beyond the polyadenylation site
Figure 18.17 Determining the guanine nucleotide exchange properties of EF-Ts
Figure 13.23 Interactions between HDAC2, Sin3A and Mad1
Figure 15.11 Effect of polyadenylation on recruitment of mRNA to polysomes
Finding DNase hypersensitivity sites. Figures 13.19 and 13.20
Figure 17.8 GTP hydrolysis is not involved in binding IF2 and fMet-tRNA to the 30S subunit
Figure 11.5 TBP Binds Minor Groove
Figure 10.27 and 10.28 Enhancers can be downstream, flipped and moved
Figures 13.15 to 13.17 Using electron microscopy to indentify nucleosome free regions in SV40
Figure 15.14 Evidence to show that the polyadenylation signal is important for polyadenylation.
Fig 6.42 complementary binding and rho independent termination
Rho Dependant Termination--Figures 6.44 and 6.45
Figure 14.11-14.13 Evidence that U1 snRNA binds to the 5' splice site
Figures 12.18-12.20 Catenane experiment to show enhancers likely function by looping.