RNA processing

This lecture explains about the RNA processing in eukaryotes including 5 prime capping and 3 prime polyadenylation.
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In 1977, work by using the Sharp and Roberts labs printed that genes of larger organisms are "cut up" or reward in a few specific segments alongside the DNA molecule.The coding regions of the gene are separated through non-coding DNA that isn't involved in protein expression. The cut up gene constitution was located when adenoviral mRNAs have been hybridized to endonuclease cleavage fragments of single stranded viral DNA. It was once located that the mRNAs of the mRNA-DNA hybrids contained 5' and 3' tails of non-hydrogen bonded regions. When greater fragments of viral DNAs have been used, forked buildings of looped out DNA have been observed when hybridized to the viral mRNAs. It was realized that the looped out areas, the introns, are excised from the precursor mRNAs in a system Sharp named "splicing". The cut up gene structure was subsequently located to be fashioned to most eukaryotic genes. Phillip Sharp and Richard J. Roberts have been awarded the 1993 Nobel Prize in Physiology or remedy for their discovery of introns and the splicing system.
Animation source: Discover Biology, Core 3rd Edition, W W Norton and company
Link- http://www.wwnorton.com/college/biology/discoverbio3/core/content/index/animations.asp

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