Molecular Biology | DBT JFR Mapping | Lec-3 | IFAS

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⏱️ Timestamps - Jump to Your Topic:
00:00:00 – Introduction
00:06:00 – DNA Polymerase: 3' OH Requirement
00:07:30 – ddNTPs & AZT (Chain Terminators)
00:17:00 – PYQ: 3' OH for Phosphodiester Bond
00:19:00 – PYQ: Origins are AT-rich (Not CG)
00:21:00 – DNA Repair: BER (Glycosylase, AP Endo)
00:28:00 – NER: Uvr (Prok), XP (Euk)
00:32:00 – Mismatch Repair & Hemimethylation
00:45:00 – PYQ: Xeroderma Pigmentosum → NER Defect
00:47:00 – Supercoiling & Topoisomerases
00:54:00 – PYQ: PCR has No Topoisomerase (Heat Denatures)
01:00:00 – Transcription Factor Specificity (Cofactors)
01:18:00 – Polycistronic (Prok) vs Monocistronic (Euk)
01:30:00 – Codon Degeneracy: AUG (Met), UGG (Trp)
01:35:00 – Frameshift Mutations (1-2 bp → Major Changes)
01:43:00 – Lac Operon: Repressor, Operator, IPTG
01:55:00 – Diauxic Growth (Glucose first)
01:59:00 – PYQ: Merodiploid – Which is NOT Inducible?
02:17:00 – Complete Lecture

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