Cell Nuclei Protein Extraction

( http://www.abnova.com ) - Nuclear protein fractionation is proposed as a method to be carried out according to the solubility of proteins in buffers of increasing ionic strength. This protocol indicates the procedure for the preparation of nuclear extracts. First, the cells are collected. Then, make cell membrane fragile using a pestle in hypotonic buffer. After collection of the cytoplasmic fraction, the nuclei are lysed and the nuclear proteins are solubilized in the lysis buffer. More videos at Abnova http://www.abnova.com

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