RBCs in Hypotonic, Isotonic and Hypertonic solution Practical Lab

Check out my other experiments! Click on HASHTAGS above the video title or the links given below to watch my other related videos.
Total WBC Count: https://www.youtube.com/watch?v=xyhbIPSLBsA&t=364s
Total RBC Count: https://www.youtube.com/watch?v=0f9p9JX4qJk&t=130s
DLC: https://www.youtube.com/watch?v=VFKm_kMTf50
Haemocytometer Use: https://www.youtube.com/watch?v=7IpE33WPRrc&t=43s
Haemometer Hb estimation: https://www.youtube.com/watch?v=6CqptdZyUaU&t=45s
Blood Smear Prep and Staining: https://www.youtube.com/watch?v=KSs0SMfERuA&t=38s
RBCs in iso, hypo and hypertonic solns: https://www.youtube.com/watch?v=urYJAPGUpuw
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To study the effect of Hypotonic, Isotonic and Hypertonic solution on RBCs
Osmosis is defined as the movement of solvent molecules from a region of lower solute concentration to a region of higher concentration across a semipermeable membrane.
Osmosis plays an important role in the interaction of cells with their external fluid surroundings.
The cell membrane functions as the semi permeable membrane through which transport of solvent molecules occur between the intra and extracellular regions.
The ability of an extracellular solution to cause the movement of solvent molecules into or out of a cell is known as the Tonicity of that solution.
A solution which has a relative solute concentration same as that found within a cell, is known as an Isotonic soution.
A solution which has a relative solute concentration lower than that found within a cell, is known as hypotonic soution.
A solution which has a relative solute concentration higher than that found within a cell, is known as hypertonic soution.
When cells are placed in a isotonic solution, they remain the same as there is no net movement of solvent into or out of the cells.
However when cells are placed in a solution which is hypotonic to them, the cells will begin to swell as there is a net movement of solvent into the cells. This process in known as Endosmosis
And finally if cells are placed in a hypertonic solution, the cells will shrink as there is a net movement of solvent from the cells to their external surrounding. This process in known as Exosmosis
In this experiment, we’re gonna make use of an animal cell, namely human red blood cells to demonstrate the effects of the three types of tonic solutions on cells.
For this experiment, we’ll need the following
• Hypo, iso and hypertonic solutions of either sodium chloride or Glucose.
• Glass slides and coverslips
• Cotton and rubbing alcohol
• A pricking device or a blood lancet
• 3 droppers
• 3 mixing sticks or toothpicks
• A compound light microscope

Begin the experiment by taking 3 clean and dry glass slides along with the three different tonic solutions with a dropper in each bottle
Clean a finger with alcohol and prick it using a lancet or pricking device.
Place a drop of blood in the centre of each slide.
Place a drop of hypotonic, isotonic and hypertonic solution on the drops of blood in each of the three slides.
Mix each drop of blood and tonic solution using 3 different mixing sticks or toothpicks.
Cover them one by one using coverslips
Allow the slides to stand for a few minutes
Observe each slide under the microscope.
In the slide containing the isotonic solution, the rbcs do not exhibit significant chance in their cell shape or volume. They retain their normal turgidity.
The rbcs exposed to hypotonic solution became swollen to such an extent that many of them could no longer retain their cellular integrity and have ruptured. The slide shows a murky appearance with only few swollen cells visible. These ruptured rbcs are commonly referred to as rbc ghosts
In the slide containing the hypertonic solution, rbcs shrink in size and exhibit a phenomenon known as RBC crenation, wherein the cells exhibit spiky appearance on their surfaces.
This simple demonstration shows the effect of solutions of varying solute concentrations on cells.
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Видео RBCs in Hypotonic, Isotonic and Hypertonic solution Practical Lab канала ThomasTKtungnung