MIT CompBio Lecture 09 - Three Dimensional Genome

MIT Computational Biology: Genomes, Networks, Evolution, Health
Prof. Manolis Kellis
http://compbio.mit.edu/6.047/
Fall 2018

Lecture 09 - Three Dimensional Genome

1. Methods for studying nuclear genome organization
- Measuring locus-landmark interactions: ChIP, DamID
- Measuring locus-locus interactions: 3C, 4C, 5C, Hi-C, ChIA-PET
2. Processing and normalization of Hi-C datasets
- Correcting for biases and issues with Hi-C data
- Normalization, sources of bias and bias correction
3. Interpretation of data and functional characterization
- Lamina Associated Domains (LADs)
- Topologically Associated Domains (TADs) and other compartments
4. Conservation of nuclear genome organization
- Across species, across cell types
- Sequence basis for conservation?
5. Towards a mechanistic understanding
- Nuclear genome organization through the (single) cell cycle
- (Limitations of) 3D modeling of nuclear genome organization
- Loop extrusion as a potential mechanism for nuclear organization

Slides for Lecture 9:
https://stellar.mit.edu/S/course/6/fa18/6.047/courseMaterial/topics/topic2/lectureNotes/Lecture09_ThreeDimensionalGenome/Lecture09_ThreeDimensionalGenome_6up.pdf

Видео MIT CompBio Lecture 09 - Three Dimensional Genome канала Manolis Kellis