Separation of Pigments of Leaves and Flowers by -- Chromatography

Aim:
To separate the pigments present in leaves and flowers by paper chromatography and determine their Rf values.
The distribution occurs in a definite ratio which represents the characteristic distribution coefficient of the solution.

The Rf coefficient ration is given by

Rf = distancetravelledbysolute
distancetravelledbysolvent

Different substances possess different Rf values. Rf depends upon a number of factors.

a)Nature of the substance
b)Nature of the solvent
c)Temperature
d)Presence of impurities
e)Quality of the filter paper
If the compound is colored, it can be easily located on the chromatographic paper. If the substance is colorless, however, a reagent may be used to treat it, which gives it a characteristic color. The name developer is given to this reagent. Iodine is the most frequently used paper chromatography developer.

Видео Separation of Pigments of Leaves and Flowers by -- Chromatography канала Santosh Kumar