Multiplicity of Infection (MOI): What is it and how do I calculate it?
So you’ve packaged your DNA into a virus and you’re ready to infect your cells! But, how many viral particles are required for 100% infection? In this video, we’ll explain the concept of Multiplicity of Infection or MOI, how to calculate your theoretical MOI, and how to determine the optimal MOI for your actual experiment.
The multiplicity of infection is defined as the ratio of how many viruses you need per cell to achieve 100% infection. Although the MOI calculation is simple, there are many factors that can affect your true experimental MOI, such as the cell and virus you are using as well as the application. We recommend that you perform a pilot experiment with, for example, a GFP-virus with your cell to determine the MOI that is optimal for your particular experiment.
Read our blog post on Multiplicity of Infection (MOI):
➜ https://info.abmgood.com/multiplicity-of-infection-moi
To learn more about the lentivirus, AAV, or Adenovirus systems, visit our knowledge base:
➜ https://info.abmgood.com/learning-resources
Connect with us on our social media pages to stay up to date with the latest scientific discoveries:
➜ Facebook: https://www.facebook.com/appliedbiological.materials/
➜ Twitter: https://twitter.com/abm_good
➜ LinkedIn: https://www.linkedin.com/company/applied-biological-material
➜ Instagram: https://www.instagram.com/abm_good
Видео Multiplicity of Infection (MOI): What is it and how do I calculate it? канала Applied Biological Materials - abm
The multiplicity of infection is defined as the ratio of how many viruses you need per cell to achieve 100% infection. Although the MOI calculation is simple, there are many factors that can affect your true experimental MOI, such as the cell and virus you are using as well as the application. We recommend that you perform a pilot experiment with, for example, a GFP-virus with your cell to determine the MOI that is optimal for your particular experiment.
Read our blog post on Multiplicity of Infection (MOI):
➜ https://info.abmgood.com/multiplicity-of-infection-moi
To learn more about the lentivirus, AAV, or Adenovirus systems, visit our knowledge base:
➜ https://info.abmgood.com/learning-resources
Connect with us on our social media pages to stay up to date with the latest scientific discoveries:
➜ Facebook: https://www.facebook.com/appliedbiological.materials/
➜ Twitter: https://twitter.com/abm_good
➜ LinkedIn: https://www.linkedin.com/company/applied-biological-material
➜ Instagram: https://www.instagram.com/abm_good
Видео Multiplicity of Infection (MOI): What is it and how do I calculate it? канала Applied Biological Materials - abm
Показать
Комментарии отсутствуют
Информация о видео
2 ноября 2019 г. 3:45:36
00:05:22
Другие видео канала
Gene therapy using adeno associated virusThe Basics of the Recombinant Lentivirus SystemLentivirus Titration: How to Determine Viral TiterGene Silencing Methods: CRISPR vs. TALENs vs. RNAiThe Immune System Explained I – Bacteria Infection1) Cell Culture Tutorial - An IntroductionThe Basics of Lentivirus Production/Packaging: Protocol, Tips, and more!How a long-forgotten virus could help us solve the antibiotics crisis | Alexander BelcrediLentivirus production - packaging, titer-measuring and stabilizingVirus Watch: Counting Viruses2) Adeno Associated Virus (AAV) - Production and Modification of AAVAssay of viral infectivityEstimating the multiplicity of infection of P. falciporum malaria parasitesMultiplicidad de infección (MOI)How to perform a CRISPR Knockin ExperimentNext Generation Sequencing 2: Illumina NGS Sample Preparation - Eric Chow (UCSF)Conditional gene expression using the Cre Lox FLEx vector switch!COVID-19 Symposium: Entry of Coronavirus into Cells | Dr. Paul Bates2) Cell Culture - Recombinant Adenovirus Expression SystemReed Muench