Maintaining focus at greater depths

Because PFS can maintain focus at greater depths within the specimen, whole images of intersegmental vessels sprouting upward from the dorsal aorta are clearly captured. Shown in the three channels are three different timepoint volumes (red: 0 mins, green: 110 mins, blue: 240 mins).
Specimen: vasculature of a zebrafish embryo (95-186 µm away from the coverslip).
Movie courtesy of: Dr. Robert Fischer, Marine Biological Laboratory

Видео Maintaining focus at greater depths канала NikonInstrumentsComp