(EMSA): Study of RNA-Protein Interactions: The IRE/IRP Example

Watch the Full Video at https://www.jove.com/v/52230/electrophoretic-mobility-shift-assay-emsa-for-study-rna-protein?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022.

Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example - a 2 minute Preview of the Experimental Protocol

Carine Fillebeen, Nicole Wilkinson, Kostas Pantopoulos
Jewish General Hospital, Lady Davis Institute for Medical Research; McGill University, Department of Medicine;

Here we present a protocol to analyze RNA/protein interactions. The electrophoretic mobility shift assay (EMSA) is based on the differential migration of RNA/protein complexes and free RNA during native gel electrophoresis. By using a radiolabeled RNA probe, RNA/protein complexes can be visualized by autoradiography.

Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts.

JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.

Subscribe to our channel: https://www.youtube.com/c/JoVEJournalofVisualizedExperiments

Видео (EMSA): Study of RNA-Protein Interactions: The IRE/IRP Example канала JoVE (Journal of Visualized Experiments)