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Agarose Gel Electrophoresis of DNA fragments amplified using PCR

This video is the third lesson in a series of resources detailing the PCR process and surrounding activities. It shows how to analyse a DNA sample using agarose gel electrophoresis, as well as how to make the agarose gel.

The following links are for similar materials to those used throughout the three stages of this tutorial. These items are not identical to all those used in the video, but should warrant similar results.

0.9% Saline Solution: https://amzn.to/2xQCoZO
High precision pipettes: https://amzn.to/2T41An5
Screw cap tubes: https://amzn.to/2YMVf32
Centrifuge: https://amzn.to/35GPUeO
Microcentrifuge tubes: https://amzn.to/3fvLlbV
Thin walled PCR tubes: https://amzn.to/3fvLlbV
PCR tube rack: https://amzn.to/2LeVtaV
PCR Cycler: https://amzn.to/2LdSWhu

Agarose Gel creation:
50x TAE buffer solution: https://amzn.to/3cn79og (this will need turning into a 1x concentration)
Agar powder: https://amzn.to/3bbtp2Q
Electrophoresis tray and system: https://amzn.to/2WkdBqx

Видео Agarose Gel Electrophoresis of DNA fragments amplified using PCR канала Schools Project
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29 марта 2015 г. 19:35:23
00:07:43
Яндекс.Метрика