Lab procedure - Fixation and Processing | Pathology | MBBS 2nd Year | Pathoclinics Series | PW

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About the Video :-
Welcome to another essential session of the Pathoclinics Series with Dr. Ranjith Sir. In this video, we discuss an important Lab Instrument: Fluorescence Microscopy. 🔬✨

Understanding the Fluorescence Microscope is crucial for Pathology Practicals and diagnosing various autoimmune and infectious diseases.

Key Learning Points:
👉🏻 Basic Principle of Fluorescence Microscopy.
👉🏻 Key components and working mechanism.
👉🏻 Diagnostic applications in Pathology (Immunofluorescence).
👉🏻 Important Viva questions for MBBS 2nd Year.
👉🏻 Master this instrument to ace your Pathology Practical Exams.
#FluorescenceMicroscopy #Pathology #LabInstruments #MBBS2ndYear #DrRanjithSir #PWMedEd #MedicalStudent #Practicals #Microscope #Immunofluorescence
00:00 - Start & Introduction
00:41 - Fixation and Processing Overview
02:03 - Need for Fixation & Hardening of Tissue
03:15 - Automated Tissue Processor Explained
04:00 - Grossing of Specimens
05:22 - Types of Fixatives (Formalin, Methanol, Glutaraldehyde)
06:38 - Frozen Section Fixative (Temperature)
08:50 - Dehydration Process using Alcohol
14:33 - Clearing Process using Xylene
17:08 - Infiltration/Impregnation with Wax
18:56 - Microtomy (Cutting Sections)
19:21 - H&E Staining Basics (Hematoxylin & Eosin)
20:03 - Quick Revision of Tissue Processing Steps
21:38 - Conclusion & Merry Christmas Wishes
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Видео Lab procedure - Fixation and Processing | Pathology | MBBS 2nd Year | Pathoclinics Series | PW канала PW MedEd