Quantification of Nucleic Acids: A260/A280 ratio, Nucleic Acid Concentration, Purity and Molarity
Any experiment requiring manipulation of a nucleic acid most likely also requires its accurate quantification to ensure optimal and reproducible results. The nitrogenous bases positioned along a nucleic acid strand absorb ultraviolet (UV) light at a wavelength of 260 nm;
at this wavelength, light absorption is proportional to nucleic acid concentration. This relationship is so well characterized that UV absorption is used to accurately determine the concentration of nucleic acids in solution.
The relationship between DNA concentration and absorption is linear up to an absorption at 260 nm (A260) of 2. For measuring the absorption of a nucleic acid solution in a spectrophotometer, most molecular biology laboratories will use quartz cuvettes with a width through which the light beam will travel 1 cm.
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Видео Quantification of Nucleic Acids: A260/A280 ratio, Nucleic Acid Concentration, Purity and Molarity канала Bio-Resource
at this wavelength, light absorption is proportional to nucleic acid concentration. This relationship is so well characterized that UV absorption is used to accurately determine the concentration of nucleic acids in solution.
The relationship between DNA concentration and absorption is linear up to an absorption at 260 nm (A260) of 2. For measuring the absorption of a nucleic acid solution in a spectrophotometer, most molecular biology laboratories will use quartz cuvettes with a width through which the light beam will travel 1 cm.
For Queries: mailtobioresource@gmail.com
Twitter:Me@BioResource
Related Videos:
---------------------------------------------------------------------------------------------------------------
Plotting Bacterial Growth Curve: https://youtu.be/NT4i6KwV6Cc
Colony Forming Units: https://youtu.be/jdnOjoOO6qY
Serial Dilution Methods: https://youtu.be/g2XdGLXcajM
Copy Number Calculation for qPCR: https://youtu.be/iKbrfj3ToxU
How to analyze Primer Sequence or Oligonucleotide sequence designed for PCR / qPCR: https://youtu.be/SqFz1Y_Z4L4
How to generate qPCR standard curve in excel and calculate PCR efficiency: https://youtu.be/pz54_p20lrI
Real Time qPCR optimization, Calculating PCR Efficiency: https://youtu.be/asUMZBFoS6A
Taqman Assay Vs SYBR Green Assay: https://youtu.be/14H5FyDCBAs
Resolving poor PCR efficiency: https://youtu.be/H8aP7AWlPa0
Calculating molecular weight of Unknown protein https://youtu.be/mCiCiO0cfbg
Designing qPCR Primers
https://youtu.be/tGNPkiY0WJU
Oligonucleotide Preparation & Purification https://youtu.be/LvyFZo3EaRk
How to check Oligo Concentration
https://youtu.be/-13riOgy0qU
Видео Quantification of Nucleic Acids: A260/A280 ratio, Nucleic Acid Concentration, Purity and Molarity канала Bio-Resource
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