Next Generation Sequencing 3: Purifying DNA Samples with Magnetic Beads - Eric Chow (UCSF)
https://www.ibiology.org/techniques/next-generation-sequencing
Next generation sequencing allows DNA samples to be sequenced quickly and affordably. Learn how next gen sequencing works and get tips on preparing and running your samples.
In the past decade there has been an amazing change in the efficiency of DNA sequencing. Using traditional Sanger sequencing, the human genome project took 20 years and cost $3 billion. Current next generation sequencing methods allow a human genome to be sequenced for $1000, in 48 hours! In this talk, Eric Chow explains the chemistry behind next generation sequencing, and describes how the next gen sequencers detect and display results. The most commonly used Illumina sequencers are image based and detect the addition of fluorescently labelled nucleotides. Chow also describes two different next generation sequencing technologies which provide benefits such as much longer reads but with downsides such as higher error rates. Chow finishes the talk with some insights into medical applications of next gen sequencing such as much less invasive prenatal testing or cancer detection.
In two short how-to videos, Chow gives advice on purifying DNA samples using magnetic beads and on determining the quality of your nucleic acid sample using an Agilent Bioanalyzer.
Speaker Biography:
Eric Chow is an assistant professor in the Department of Biochemistry and Biophysics and the Director of the Center for Advanced Technology (CAT) at the University of California, San Francisco. The CAT provides resources for UCSF labs wishing to use next generation sequencing techniques and Chow’s research program strives to develop new applications for NGS in pathogen diagnostics. Chow received his BA in molecular biology from the University of California, Berkeley and his PhD in biochemistry from UCSF.
Видео Next Generation Sequencing 3: Purifying DNA Samples with Magnetic Beads - Eric Chow (UCSF) канала iBiology Techniques
Next generation sequencing allows DNA samples to be sequenced quickly and affordably. Learn how next gen sequencing works and get tips on preparing and running your samples.
In the past decade there has been an amazing change in the efficiency of DNA sequencing. Using traditional Sanger sequencing, the human genome project took 20 years and cost $3 billion. Current next generation sequencing methods allow a human genome to be sequenced for $1000, in 48 hours! In this talk, Eric Chow explains the chemistry behind next generation sequencing, and describes how the next gen sequencers detect and display results. The most commonly used Illumina sequencers are image based and detect the addition of fluorescently labelled nucleotides. Chow also describes two different next generation sequencing technologies which provide benefits such as much longer reads but with downsides such as higher error rates. Chow finishes the talk with some insights into medical applications of next gen sequencing such as much less invasive prenatal testing or cancer detection.
In two short how-to videos, Chow gives advice on purifying DNA samples using magnetic beads and on determining the quality of your nucleic acid sample using an Agilent Bioanalyzer.
Speaker Biography:
Eric Chow is an assistant professor in the Department of Biochemistry and Biophysics and the Director of the Center for Advanced Technology (CAT) at the University of California, San Francisco. The CAT provides resources for UCSF labs wishing to use next generation sequencing techniques and Chow’s research program strives to develop new applications for NGS in pathogen diagnostics. Chow received his BA in molecular biology from the University of California, Berkeley and his PhD in biochemistry from UCSF.
Видео Next Generation Sequencing 3: Purifying DNA Samples with Magnetic Beads - Eric Chow (UCSF) канала iBiology Techniques
Показать
Комментарии отсутствуют
Информация о видео
Другие видео канала
Next Generation Sequencing 4: Checking Nucleic Acids with an Agilent BioAnalyzer - Eric Chow (UCSF)Next Generation Sequencing 1: Overview - Eric Chow (UCSF)Illumina Sequencing Overview: Library Prep to Data Analysis | Webinar | Ambry GeneticsSingle Cell Sequencing - Eric Chow (UCSF)How to isolate total RNA using magnetic bead–based technologyThe Workflow | Ion S5 Next Generation Sequencing SystemW16: Library Prep for NGS- Day 3Next Generation Sequencing 2: Illumina NGS Sample Preparation - Eric Chow (UCSF)Life in the Lab: A DNA sequencing pipelineMagnetic bead based DNA/RNA isolation with chemagen TechnologySureSelect: AMPure Beads PurificationDNA extraction from BloodHow is Oxford Nanopore used? | WIREDImageJ - Kevin Eliceiri (U. Wisconsin)Next generation sequencing for the clinical oncologist: Demystifying the genomics black boxSanger sequencing2) Next Generation Sequencing (NGS) - Sample PreparationGuide to magnetic beads / MagBeads for protein purificationHow does a DNA sequencing machine work?Illumina Sequencing by Synthesis