Libermann-Burchard's test: Part 2 (Identification of Cholesterol)
Principle:
Cholesterol as a derived lipid is a member of the group of steroid known as sterols. Imbalance in lipid metabolism can lead to major clinical problems such as obesity and atherosclerosis.
Method:
- 2 ml of cholesterol dissolved in chloroform,
- Add 2 mL of acetic anhydride + 5 drops of concentrated sulfuric acid;
- A bluish-green colour is formed indicating the presence of cholesterol
Видео Libermann-Burchard's test: Part 2 (Identification of Cholesterol) канала Dr. Mag
Cholesterol as a derived lipid is a member of the group of steroid known as sterols. Imbalance in lipid metabolism can lead to major clinical problems such as obesity and atherosclerosis.
Method:
- 2 ml of cholesterol dissolved in chloroform,
- Add 2 mL of acetic anhydride + 5 drops of concentrated sulfuric acid;
- A bluish-green colour is formed indicating the presence of cholesterol
Видео Libermann-Burchard's test: Part 2 (Identification of Cholesterol) канала Dr. Mag
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