Transcriptomics 1: Introduction to RNA-Seq

This course is an introduction to Next Generation Sequencing for the study of RNA expression. Topics include quantitative and qualitative analysis of RNA in a sample, data preparation using Next Generation Sequencing and preparation of a table of expression from raw FASTq files. Visualization of high-dimensional data using Principal Component Analysis (PCA).

Mapping raw reads to reference genome and transcriptome
Detecting junctions and assembly of isoforms
Quantification of mRNA: Gene, isoform and exon expression table

https://edu.t-bio.info/course/transcriptomics-1/

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