How to calculate Relative gene expression in qPCR using Delta Delta Ct method | Livak Method

🧬 Video Highlights:

Introduction to qPCR: A brief overview of the qPCR technique and its significance in quantifying gene expression levels with high precision.

Understanding Ct Values: Explore the concept of Ct values and their role in qPCR, laying the foundation for the subsequent calculations in the Delta Delta Ct method.

Delta Ct Calculation: Learn the first step of the Livak method - calculating the Delta Ct, which involves comparing the target gene Ct values with those of a reference gene or control sample.

Normalization: Understand the importance of normalization in gene expression analysis and how it enhances the accuracy of results.

Delta Delta Ct Calculation: Dive into the core of the Livak method by calculating the Delta Delta Ct, allowing for the comparison of gene expression between experimental and control groups.

Interpretation of Results: Gain insights into interpreting the Delta Delta Ct values and how they correlate with changes in gene expression levels.

Practical Tips and Troubleshooting: Receive valuable tips for optimizing your qPCR experiments and troubleshooting common issues that may arise during the analysis.

#qPCR #GeneExpressionAnalysis #MolecularBiology #LivakMethod #DeltaDeltaCt #ScienceTutorial #ResearchMethods #LabTechniques

Видео How to calculate Relative gene expression in qPCR using Delta Delta Ct method | Livak Method канала Applied Biology