Getting The Most Out Of Your LCMSMS Separations and Method Development

Presenter: Rick Lake, Director of Business Development, Restek

LC-MS/MS is changing the role of chromatography. Historically, when utilizing LC with traditional detectors, like UV, the chromatographer was looking for complete analyte separation, with the ability to optimize mobile phases as needed. Today, when utilizing LC-MS/MS, we are often looking to chromatography to assist the MS in achieving higher sensitivity, getting greater peak capacity and provided selectivity where needed, like isobaric separations. In addition, the number of amenable mobile phases has been reduced, as they must be volatile. In this presentation, we will first discuss the needs of chromatography for LC-MS/MS, beginning with the fundamental concepts and how we can apply them. We will then discuss options for selecting the best column formats for LC-MS/MS as it relates to the needs of MS. Lastly, we will review a quick approach to developing a separation when using LC-MS/MS. We will focus on a small, versatile set of column chemistries that work well with MS and how we can effectively adjust a small set of MS amenable mobile phases to work with these chemistries.

In short, we will define a novel approach to simplifying chromatography for LC-MS/MS and how we can best utilize chromatography to make the LC-MS/MS work better.

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Rick has been actively working in the field of chromatography for nearly 20 years. Currently, Rick is the Director of Business Development where he manages the coordination of applications and collaborations that serve multiple industries. Previously, he was Product Line Manager, overseeing the development and application of Restek's LC products. Prior to this, Rick served as our Pharmaceutical Market Development Manager and Pharmaceutical Innovations Chemist, developing GC and LC applications and assisting in new product development. Before coming on board in 2005, he worked for 7 years as a Study Director and Principal Investigator for a contract research lab, where he developed and validated GC and LC methods while also managing the Stability Testing Department. Prior to shifting his focus to pharmaceuticals, he spent 4 years performing pesticide residue and volatile/semivolatile analyses.

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