Dr. Talya Dayton "Organoid models of neuroendocrine cell growth and tumorigenesis"

Organoid cultures are a powerful model system for the study of cell biology and human disease. We have applied the organoid culture system to study rare cell types and diseases, with a focus on neuroendocrine (NE) cells and tumors. A paucity of in vitro and in vivo models has limited the study of Neuroendocrine neoplasms (NENs). Moreover, the presumed precursor cells for this tumor type, normal neuroendocrine (NE) cells are exceedingly rare and have been difficult to culture. We have generated the first living biobank of patient derived organoids (PDOs) from low- and high-grade NENs from a number of different tissue sites. In parallel, to study normal NE cells of the lung, we have established organoid protocols that allow for the generation of large numbers of these, normally very rare, differentiated cells in vitro. These novel organoid culture systems are being used for further molecular and genetic analyses and will be used in combination with CRISPR/Cas9 technology to model the genesis and progression of NENs.

Видео Dr. Talya Dayton "Organoid models of neuroendocrine cell growth and tumorigenesis" канала The London Stem Cell Network