Semen Analysis Practical Lab | Sperm Motility Test

Do watch the rest of my semen analysis videos by clicking on below links:
Semen analysis Complete Video: https://www.youtube.com/watch?v=OMRxQgCl6rw&t=109s
Semen Analysis 1-6 Liquefaction time, Volume, Colour, pH, Odour, Viscosity: https://www.youtube.com/watch?v=POjLFOiaoqs&t=33s
Semen analysis Sperm Motility Test: https://www.youtube.com/watch?v=a3zB7ksgUbU
Semen analysis Sperm Viability Test: https://www.youtube.com/watch?v=tdm61DfCUqo
Semen Analysis Sperm Morphology: https://www.youtube.com/watch?v=EV4QL9ZnSMM&t=160s
Semen analysis Sperm Count: https://www.youtube.com/watch?v=7dkzPNzTUtE&t=17s
Semen analysis Fructose content: https://www.youtube.com/watch?v=y7-RJ1uUFgA&t=2s
Eosin and Nigrosin Stain Preparation (for Viability Test): https://www.youtube.com/watch?v=czwD3UNiE1U&t=91s

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Sperm motility test is done within 60mins of sample collection. Sperm motility is the ability of a sperm cell to move in the surrounding fluid medium.
A drop of liquefied semen sample is taken in a glass slide and gently covered with a coverslip. The slide is immediately observed under 40x objective of the microscope.
100 or 200 random sperm cells are briefly observed one by one to check for motility or the lack thereof.
To minimise the chances of repeated assessment of the same sperm cell for motility, the microscopic field of view is changed frequently in a specific order of movement such as from the left corner of the slide to the right corner etc until 200 cells have been assessed.
Based on the extent of motility, Human sperms are divided into four grades also known as Motility grades. The various grades of motility are calculated in terms of percentage by counting 200 random sperm cells
• Grade A, Motility IV. Rapid Progressive Motility. These are the strongest and swim fast in a straight line.
• Grade B: Motility III. Slow/Sluggish/Non-Linear Progressive Morility These also move forward but tend to travel in a curved or crooked motion.
• Grade C: Motility II. Non-progressive motility. They move their tails but do not move forward.
• Grade D: Motility I. Immotility. These are immotile and fail to move at all.

Normal semen contains atleast 50% Grade A+B sperm cells combined
Low Sperm motility of below 50% , also known as Asthenospermia, can be a symptom of disorders related to testicular function and male accessory sex glands. It could also be a sign of infection or injury in the male reproductive tract.
In cases of reduced sperm motility, the semen sample should also be checked for sperm viability.

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