3) Polymerase Chain Reaction (PCR) - Quantitative PCR (qPCR)

What is Quantitative PCR (qPCR)?
➜ Real-Time PCR or quantitative PCR (qPCR) is a PCR-based technique that is able to simultaneously amplify and detect changes in the amplicon concentration. Real-time PCR collects data during PCR amplification by utilizing fluorescence signals emitted by either special probes or DNA binding dyes.

For more information on qPCR and for a list of the sources used, please visit:
➜ Knowledge Base: https://info.abmgood.com/polymerase-chain-reaction-pcr

**Please note that the labels of 5’ and 3’ are flipped at 4:35 – 5:22. Usually R (reporter) is at the 5' end and Q (quencher) is at the 3' end, and thus the top strand bound by the Taqman Probe should be labelled as 3’ on the left, and 5’ on the right. Vice versa for the bottom strand: this should be labelled as 5’ on the left, and 3’ on the right. Please refer to Figure 3 of our knowledge base: https://bit.ly/2Xwdybj. We apologize for the confusion and thank our youtube community for pointing out the error.

Thank you to all of the amazing community contributions towards translating this video into other languages including:
➜ Indonesian
(To view the translations, toggle the CC button and then go to Settings ➜ Subtitles/CC to select a language.)

Check out our other video series:
➜ Polymerase Chain Reaction (PCR) - An Introduction: https://youtu.be/matsiHSuoOw
➜ CRISPR Cas9: https://youtu.be/1aJxXWkE3Ek?list=PLTt9kKfqE_0Ei8_rQsrfm01-zQtABTn0Z
➜ Adeno Associated Virus: https://youtu.be/hYHbfQe5h-Q?list=PLTt9kKfqE_0HfXQMX9RPgbcmSWMqVtlBf
➜ Cell Culture: https://www.youtube.com/playlist?list=PLTt9kKfqE_0HZg-0pqGKk9Pr-mc_Gi4WX

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Видео 3) Polymerase Chain Reaction (PCR) - Quantitative PCR (qPCR) канала Applied Biological Materials - abm