Step-by-Step Plant RNA Extraction Protocol | Laboratory Guide

Step inside the molecular biology lab for a complete, step-by-step demonstration of a plant total RNA extraction protocol.

In this video, we guide you through the practical workflow of isolating high-quality RNA from fresh plant leaf tissue. From sample homogenization in liquid nitrogen to phase separation, washing, and final elution, you'll see every critical benchwork step using standard laboratory equipment like microcentrifuges, vortex mixers, and precision pipettes.

🔬 Key Steps Covered:
0:00 - Harvesting and preparing the plant leaf tissue
0:05 - Homogenization / grinding the sample
0:08 - Adding RNA extraction lysis buffer
0:15 - Chloroform phase separation and centrifugation
0:25 - Precipitating and washing the RNA pellet
0:45 - Rehydrating / eluting the final total RNA sample

Whether you are a student learning molecular techniques, a researcher refining your extraction yields, or a science enthusiast curious about biochemistry workflows, this bench-side walkthrough has you covered.

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Видео Step-by-Step Plant RNA Extraction Protocol | Laboratory Guide канала Chem Tech