How I Defeated My Arch Nemesis

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BL21 E. coli are by far the most obnoxious bacteria genetic engineers usually work with for 1 simple reason. They are maddeningly difficult to modify and load with new DNA. This wouldn't be an issue if they weren't also one of the best types for producing proteins en mass, and as such have application in everything from pharmaceutical production, to producing ingredients for foods. But after a few years of tinkering with it I finally found a protocol that reliably fixes this irritating issue, and makes it easy to transform them reliably.

Written protocol - https://github.com/thethoughtemporium/Protocols/blob/main/Transformations/bl21%20transformation.md

Sebastian's spinner - https://www.tinkercad.com/things/5t5GqlGkK1i

Previous videos;
Modifying Bacteria - https://youtu.be/F0_q-fD_lyU
Modifying yeast - https://youtu.be/DHNPnO5UOYQ
Making a heat block/incubator - https://youtu.be/Zm378x6zJYc
Growing spider silk -https://youtu.be/2hf9yN-oBV4
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Видео How I Defeated My Arch Nemesis канала The Thought Emporium