SGPT (ALT) Test Trick 🔥 | Liver Test in 30 Sec #sgpttest

SGPT ya ALT (Alanine Aminotransferase) ek liver-specific enzyme hai jo liver damage hone par blood me increase ho jata hai. Iska principle bhi transamination reaction par based hai jisme L-alanine aur alpha-ketoglutarate react karke pyruvate aur glutamate banate hain.
Pyruvate NADH ke saath react karta hai (LDH enzyme ke through) aur lactate me convert hota hai. Is process me NADH decrease hota hai aur absorbance kam hoti hai, jise 340 nm wavelength par measure kiya jata hai.
Yeh test kinetic UV method se kiya jata hai aur absorbance ke decrease ka rate ALT activity batata hai.
Normal values:
Male: 7–56 IU/L
Female: 5–40 IU/L
ALT liver damage ka best indicator mana jata hai aur exams me frequently pucha jata hai. Short revision ke liye perfect concept 🚀
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