How to make malt yeast agar plates for mushroom cultivation. Using a Still Air Box.

In this video I show you how to mix up some malt yeast agar, pour it into glass plates(petri dishes) inside a still air box. This is a fundamental part of mushroom cultivation as it allows you to germinate spores, grow out cultures, isolate strains and make clones etc.

Items needed:
* Glass or plastic plates (Petri Dishes)
* A glass bottle
* Some Polyfil
* Weighing scales
* A measuring jug
* A spoon
* A pressure cooker
* A still air box (Explained in video)/ Or a flow hood, if you have one.

* Rubber gloves
* A spray bottle filled with antibacterial soap and water
* Isopropyl alcohol
* Parafilm
Ingredients needed:
* Warm water
* Agar-Agar
* Malt Extract
* Baker's Yeast

Weigh out dry ingredients. Then combine them with warm water, stir thoroughly and then pour solution into your glass bottle.

Load glass bottle into your pressure cooker, bring to the boil/full pressure. Then leave at full pressure for 45 minutes.

Once you're finished sterilizing the mixture. Leave your pressure cooker to cool for about 1.5-2 hours. This should be enough time so that the agar has cooled enough so that you can handle it but not cool enough so that it's set.

Prepare your still air box by spraying it down with your antibacterial soap and water solution. Then wiping it down with isopropyl alcohol. Load your plates and bottle containing your malt yeast agar solution into your still air box. Wiping them down with isopropyl alcohol also, before placing them inside.

Unscrew the lid of your bottle then pour plates quickly and smoothly, limiting the amount of time that they are exposed to the air.

Once all of your plates are poured, leave them to cool for about 20-30 minutes. While they are cooling cut some pieces of Parafilm.

Pull the parafilm tight across the lid and bottom part of the plate. You can store these plates in a cold dark place or go ahead and use them right away for whatever cultures or stuff you want to grow out.

If you found this video useful, feel free to hit the like button and/or subscribe to this channel for notifications when I upload new content.

Видео How to make malt yeast agar plates for mushroom cultivation. Using a Still Air Box. канала Things and stuff like that