Edman degradation | Edman Sequencing
Edman degradation is a a method used for the sequencing of peptide/protein. In this method the peptide is allowed to react with edmans reagent under mild alkaline conditions. The edmans reagent reacts with
the N terminal amino acid and forms a complex. This complex is then cleaved from the peptide and separated by chromatography or electrophoresis. The method can be repeated several times to find the sequence of the peptide.
Видео Edman degradation | Edman Sequencing канала Quick Biochemistry Basics
the N terminal amino acid and forms a complex. This complex is then cleaved from the peptide and separated by chromatography or electrophoresis. The method can be repeated several times to find the sequence of the peptide.
Видео Edman degradation | Edman Sequencing канала Quick Biochemistry Basics
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19 декабря 2019 г. 2:30:00
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