Advancements in Translational Biomarker Identification from Cell-Free RNA

Liquid biopsy is most often associated with cell-free DNA, but new research has shown additional value in interrogating a broader diversity of analytes in the blood.

Cell-free RNA (cfRNA) is one such molecule that has been shown to reflect dynamic changes in many tissues. Researchers at Illumina have optimized key variables to enable scalable, end-to-end workflow solutions for whole-transcriptome library preparations of cfRNA.

Continued application of cfRNA stands to benefit a multitude of human health concerns by empowering non-invasive interrogations of disease etiology and identification of predictive, diagnostic, and prognostic biomarkers, all of which will further facilitate our transition into the era of personalized medicine.

#liquidbiopsy #rna #rnaseq #sequencing #transcription

Resources:
Paper referred to in video - https://www.science.org/doi/10.1126/scitranslmed.aaz0131
RNA prep with Enrichment - https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/rna-prep-enrichment.html

Presenters:
Dan Gheba – Executive Sequencing Specialist, Illumina
Suzanne Rohrback PhD – Senior Scientist, Advanced Sciences, Illumina

00:00 Introduction to Liquid Biopsy
01:25 Why RNA over DNA?
04:24 Illumina cfRNA Study Introduction
05:43 cfRNA quality and method development
08:52 Sample Collection best practices
10:50 Study findings – unique biomarkers
14:24 Machine learning model
16:52 Method Update!
19:09 Publication Summary
20:28 How can you use cfRNA in your research?

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A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Illumina technology is responsible for generating more than 90% of the world’s sequencing data.* Through collaborative innovation, Illumina is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond.

*Data calculations on file. Illumina, Inc., 2015.

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Видео Advancements in Translational Biomarker Identification from Cell-Free RNA канала Illumina