Complete MTT Assay Analysis | % Cell Viability, SD, SEM, IC₅₀, Dose-response Graph, GraphPadPrism
🎓 This tutorial is for educational and teaching purposes only.
Learn step-by-step how to analyze MTT assay data from raw absorbance readings (570 nm – 630 nm) using Microsoft Excel and Graph making using GraphPad Prism
🔬 In this tutorial, you will learn:
How to process absorbance values (570 nm - 630 nm)
How to calculate % Cell Viability and Standard Error of Mean (SEM), SD
How to create a dose-response graph to estimate IC₅₀ values
How to plot a histogram of cell viability for visual comparison
🧪 Ideal for:
PhD students, researchers, and life science professionals analyzing cytotoxicity or drug screening data using MTT assays.
📊 Tools used:
✔ Microsoft Excel: Statistical analysis
✔ GraphPad Prism (https://www.graphpad.com/free-trial)
📌 Disclaimer: This tutorial is intended only for education, and teaching purpose only. Always validate your analysis methods for scientific publication.
🔔 Don’t forget to Like, Subscribe, and Share if you find it helpful!
#MTTAssay #graphpadprism #cytotoxicity #IC50 #PhDLife #Cytotoxicity #GraphPadPrism #BiomedicalResearch #Microsoftexcel #histogram
Видео Complete MTT Assay Analysis | % Cell Viability, SD, SEM, IC₅₀, Dose-response Graph, GraphPadPrism канала LearnBytez31
Learn step-by-step how to analyze MTT assay data from raw absorbance readings (570 nm – 630 nm) using Microsoft Excel and Graph making using GraphPad Prism
🔬 In this tutorial, you will learn:
How to process absorbance values (570 nm - 630 nm)
How to calculate % Cell Viability and Standard Error of Mean (SEM), SD
How to create a dose-response graph to estimate IC₅₀ values
How to plot a histogram of cell viability for visual comparison
🧪 Ideal for:
PhD students, researchers, and life science professionals analyzing cytotoxicity or drug screening data using MTT assays.
📊 Tools used:
✔ Microsoft Excel: Statistical analysis
✔ GraphPad Prism (https://www.graphpad.com/free-trial)
📌 Disclaimer: This tutorial is intended only for education, and teaching purpose only. Always validate your analysis methods for scientific publication.
🔔 Don’t forget to Like, Subscribe, and Share if you find it helpful!
#MTTAssay #graphpadprism #cytotoxicity #IC50 #PhDLife #Cytotoxicity #GraphPadPrism #BiomedicalResearch #Microsoftexcel #histogram
Видео Complete MTT Assay Analysis | % Cell Viability, SD, SEM, IC₅₀, Dose-response Graph, GraphPadPrism канала LearnBytez31
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7 июля 2025 г. 1:15:01
00:21:35
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