Genetically encoded chemical tools for protein studies in the live cell

Talk given by Irene Coin, PhD (Leipzig) as part of the International GCE Webinar series. Live talk given on November 17th, 2022.

We apply non-canonical amino acids (ncAAs) to address general questions about functioning of G protein-coupled receptors (GPCRs) directly from the natural environment of the live mammalian cell. On one hand, we use photo-and chemical crosslinking amino acids to define the topology of GPCR interactions both with ligands (especially peptide ligands) and intracellular partners. On the other hand, we have engineered enhanced tRNAs that have enabled efficient incorporation of last generation ncAAs for bioorthogonal chemistry into challenging protein targets. In this way, we could achieve quantitative single-residue labeling of sensitive GPCR regions, such as the loops, with small organic fluorescent probes and put the basis for the development of small-size fluorescent sensors for in-cell studies of GPCR dynamics.

Видео Genetically encoded chemical tools for protein studies in the live cell канала GCE4All Research Center