Antibiotic sensitivity testing: Kirby Bauer disk diffusion method

The Kirby-Bauer disk diffusion method, also known as the Kirby-Bauer test, is a standard procedure for determining how susceptible bacteria are to antibiotics and other antimicrobial compounds. This test helps doctors choose the best treatment options for their patients.
Here's how the Kirby-Bauer test works:
Isolate a pure culture of bacteria
Grow the bacteria on an agar plate
Add antibiotic-impregnated filter paper disks to the agar
Incubate the plate at 35°C for 24 hours
Observe the plate for a "zone of inhibition"
Measure the diameter of the zone of inhibition
Compare the zone size to a standardized chart
A zone of inhibition indicates that the bacteria are sensitive to the antibiotic. The larger the zone, the more sensitive the bacteria are. If there is no zone of inhibition, the bacteria are resistant to the antibiotic

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