Billy Ng - Chemically Induced Proximity forTargeted Protein O-GlcNAcylation

Billy Ng, Associate Professor, School of Pharmacy, Faculty of Medicine, The Chinese University of Hong Kong (CUHK) speaks on "Chemically Induced Proximity for Targeted Protein O-GlcNAcylation".

Abstract
Protein O-linked β-N-acetylglucosamine modification (O-GlcNAcylation) plays a crucial role in regulating essential cellular processes. The disruption of O-GlcNAcylation homeostasis has been linked to various human diseases, including cancer, diabetes, and neurodegeneration.
However, there are limited chemical tools for protein- and site-specific O-GlcNAc modification, rendering the precise study of O-GlcNAcylation challenging.
To address this, we have developed bifunctional small molecules, named O-GlcNAcylation TArgeting Chimeras (OGTACs), which enable protein-specific O-GlcNAcylation in living cells. OGTACs promote O-GlcNAcylation of proteins such as p53, BRD4, CK2α, and EZH2 in cellulo by recruiting O-GlcNAc transferase (OGT), with temporal, magnitude, and reversible control. Overall, OGTACs represent a promising approach for inducing protein-specific O-GlcNAcylation, thus enabling functional dissection and offering new directions for O-GlcNAc-targeting therapeutic development.

Видео Billy Ng - Chemically Induced Proximity forTargeted Protein O-GlcNAcylation канала Int'l Centre for Genetic Eng. and Biotechnology