Lowry Assay for Protein Estimation | Folin Lowry Method | Dr. Nagendra Singh | PENS#39

The Lowry protein assay for protein estimation is based on the biuret reaction with additional steps and reagents to increase the sensitivity of detection. In the biuret reaction, copper interacts with four nitrogen atoms of peptides to form a cuprous complex. Lowry adds phosphomolybdic /phosphotungstic acid also known as Folin-Ciocalteu reagent. This reagent interacts with the cuprous ions and the side chains of tyrosine, tryptophan, and cysteine amino acid residues to produce a blue-green color that can be detected between 650 nm and 750 nm. The protein detection range is 5–100 μg in this method.
The main methods of protein estimation are:
1. UV based detection
2. Biuret method
3. Lowry method
4. BCA method
5. Bradford method

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