Heat Shock Transformation with DH10B Chemically Competent E. coli Cells

Learn how to perform a Heat Shock Transformation using GoldBio’s DH10B Chemically Competent Cells.

Heat Shock Transformation (HST) is a basic molecular biology technique that allows a researcher to insert plasmid DNA into treated E. coli cells. Chemically competent cells are cells that have been treated with a salt (often CaCl2 or MgCl2). When these cells are rapidly heated and cooled, the cell membrane becomes permeable, allowing the plasmid DNA to enter the competent cells and to be further replicated.

Protocol demonstrated:

DH10B Chemically Competent E. coli Cells Transformation Protocol - http://ow.ly/uulO50yIbru

Products Mentioned:

DH10B Chemically Competent E. coli Cells - https://www.goldbio.com/product/14437/dh10b-chemically-competent-e-coli-cells

More information on GoldBio's Competent Cells - https://www.goldbio.com/collection/competent-cells

Related articles/resources:

DH10B Chemically Competent E. coli Cells Transformation Protocol - http://ow.ly/uulO50yIbru

Transformation Efficiency Calculator - https://www.goldbio.com/transformation-efficiency-calculator

GoldBio's Competent Cell Guide - http://ow.ly/qVU150yId8e

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Credits - Written By Tyasning Kroemer and Karen Pierce; Demonstrated by Tyasning Kroemer; Voice-over by Karen Pierce; Filmed and Edited by Karen Pierce

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Видео Heat Shock Transformation with DH10B Chemically Competent E. coli Cells канала Gold Biotechnology, Inc.