Lecture On Clinical Biochemistry Practical: Normal Ranges, Principles & Viva Q&A | MBBS

Prepare for your MBBS practical exams with this interactive viva session at Santiniketan Medical College & Hospital. In this class, we review essential biochemistry principles, normal ranges, and clinical correlations that every first-year medical student must know for their practical and theory examinations.

[What You Will Learn]
This session covers the "basics of basics"—the high-yield questions that frequently appear in medical school vivas.

Key Topics Discussed:

Creatinine Estimation: The Jaffe method principle (alkaline picrate) and normal ranges [00:00].

Protein & Albumin Principles: Understanding the Biuret reagent for total protein and Bromocresol Green (BCG) for albumin [02:50].

Cholesterol & Steroids: Principles of estimation and the physiological functions of cholesterol in the body [05:57].

Uric Acid & Clinical Correlates: Normal ranges (3-7 mg/dl), the degradation of purine nucleotides, and clinical conditions like Gout and Von Gierke's Disease [14:46].

Albumin Functions: Transport of unconjugated bilirubin, free fatty acids, and the maintenance of colloidal osmotic pressure [07:07].

Practical Tips: How to draw a peptide bond and understanding the copper complex in protein estimation [20:03].

[Timestamps]
00:00:10 - Creatinine & The Alkaline Picrate Method
00:01:11 - Normal Ranges for Creatinine
00:02:50 - Principles of Total Protein & Albumin Estimation
00:07:07 - Functions of Albumin in the Body (Transport & Osmotic Pressure)
00:09:42 - Understanding Pitting vs. Non-Pitting Edema
00:11:07 - Transport of Unconjugated Bilirubin & Fatty Acids
00:12:50 - Functions of Cholesterol & Steroid Hormone Synthesis
00:14:46 - Uric Acid: Normal Ranges & Purine Degradation
00:17:26 - Clinical Conditions: Gout, Von Gierke’s & Elevated Uric Acid
00:19:00 - Total Protein: The Biuret Principle
00:20:03 - Practical Demonstration: Drawing a Peptide Bond
00:24:41 - Advice for Practical Exam Success

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SANTINIKETAN MEDICAL COLLEGE & HOSPITAL
Real Classroom Demonstration/Lecture
On: BIOCHEMISTRY by Dr. Karan Pal Singh
MBBS Batch: (2025-26)
Conducted on: 08-05-26

Видео Lecture On Clinical Biochemistry Practical: Normal Ranges, Principles & Viva Q&A | MBBS канала Santiniketan Medical College